Johanna Klughammer: "Reference genome independent DNA methylation analysis. Mapping genome-wide DNA methylation patterns across the species tree"
12:00 - 13:00
Johanna Klughammer vom CeMM, Vienna, Austria
Mehr Informationen hier: http://www.medical-epigenomics.org/meg/index.php?option=com_content&view=article&id=93&catid=82&Itemid=489
Abstract (auf Englisch)
The genome-wide study of DNA methylation at
cytosines is crucial for understanding fundamental biological
processes such as animal development, cellular differentiation,
and maintenance of cellular identity. Assessing DNA methylation in
a large variety of species and in wild populations is expected to
provide insights into evolutionary processes, environmental
adaptation, and mechanisms of epigenetic regulation.
To enable cost-efficient, genome-wide, high-resolution, and
high-throughput analysis of DNA methylation in non-model organisms
and in species that lack a high-quality reference genome, we
developed an integrated experimental and computational approach
for studying DNA methylation independent of a reference genome.
Empowered by this novel approach, we now investigate the
evolutionary role of DNA methylation in the determination of
cellular identity by mapping cell type specific genome-wide DNA
methylation patterns in a large number of animal species across
the species tree.