Developmental dynamics of catshark cranial neural crest cells provide insights into gnathostome facial evolution
Cranial neural crest cells (CNCC) are a jawed vertebrate-specific, multipotent cell population central to facial morphogenesis and a cellular substrate for evolutionary change. Although core CNCC developmental programs are deeply conserved, changes in their gene expression programs and cell behaviour underlie both macroevolutionary transitions and microevolutionary adaptations. While CNCC biology has been well characterized in bony vertebrates, comparatively little is known about CNCC properties and the behavior of their derivatives in cartilaginous fishes (Chondrichthyes). To address this gap, we investigate CNCC development in a representative chondrichthyan: the small-spotted catshark (Scyliorhinus canicula). By integrating high-resolution molecular and morphological analyses, we reveal how conserved developmental programs are modulated in chondrichthyans to generate divergent facial morphologies. We show that the molecular toolkit of CNCC is largely conserved across jawed vertebrates, and the developmental divergence and lineage-specific differences arise from divergent behaviour of their ectomesenchymal derivatives. These findings establish a high-resolution reference of CNCC biology in Chondrichthyes and uncover the evolutionary origins of both shared and lineage-specific traits, offering key insights into the developmental and evolutionary processes shaping gnathostome facial diversity.
https://doi.org/10.1242/dev.205258
Interactive dataset for exploring gene expression profiles during small-spotted catshark (Scyliorhinus canicula) embryogenesis with a focus on cranial neural crest cells and facial evolution: